CD11b antibody (10R-6348)
Rat monoclonal CD11b antibody
Overview
Overview
| Synonyms | Monoclonal CD11b antibody, Anti-CD11b antibody, CR3 antibody, CD-11b antibody, CD-11b, Ma-1c antibody, CD 11b, ITGAM antibody, CD11b, integrin alpha M antibody, CD 11b antibody, Integrin alpha M antibody |
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| Specificity | Mouse |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD11b antibody (10R-6348)
Staining of mouse bone marrow with CD11b antibody (FITC) (left) (PE) (right). Appropriate Isotype Controls were used (open histogram). Cells in the myeloid population were used for anaysis.
Specifications
| Host | Rat |
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| Clone | M1/70 |
| Isotype | IgG2b kappa |
| Method of Purification | CD11b antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.25 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin and binds to CD54, C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions. |
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