CD144 antibody (20R-CR019)
Rabbit polyclonal CD144 antibody
Overview
| Synonyms | Polyclonal CD144 antibody, Anti-CD144 antibody, CD 144, CD-144, CD-144 antibody, VE-Cadherin antibody, CD144, CD 144 antibody |
|---|---|
| Specificity | Human |
| Cross Reactivity | Bovine, mouse, rat |
| Applications | ELISA, FC, IHC-F, WB |
| Immunogen | CD144 antibody was raised in rabbit using recombinant protein fragments corresponding to amino acids 1-258 of VE-cadherin as the immunogen. |
Images
Western blot analysis using CD144 antibody (20R-CR019)
Immunoblot analysis of VE-cadherin and catenin expression in endothelial cells exposed to anti-Cad2 antibody. A, total cell lysates of untreated cells (lanes 1 and 4) and of cells treated with anti-Cad2 antibody for 2 h (lanes 2 and5) or 4 h (lanes 3 and6) were electrophoresed and transferred to nitrocellulose membranes before probing with anti-VE-Cad, anti-alpha-catenin, anti-beta-catenin, and anti-plakoglobin (plako) antibodies.Lanes 4–6 correspond to cells treated with cycloheximide for 4 h prior to the addition of anti-VE-Cad.B, shown are the results from the quantification of the amounts of VE-cadherin and catenins. The amounts of VE-cadherin (panel a), alpha-catenin (panel b), beta-catenin (panel c), and plakoglobin (panel d) were estimated, using image analysis software, from the ECL bands of the Western blots described for A. Shaded bars, without cycloheximide; white bars, with cycloheximide. The relative amounts of prote
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | CD144 antibody was purified by affinity chromatography. |
| Form & Buffer | Purified IgG supplied in PBS. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
|---|
Storage & Safety
| Storage | Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles |
|---|
General Information
| Biological Significance | VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. |
|---|
Reviews
Sorry there are currently no reviews for this product