CD16/32 antibody (allophycocyanin) (61R-1710)
Rat monoclonal CD16/32 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD16/32 antibody, Anti-CD16/32 antibody, IGFR3 antibody, IGFR2 antibody, CD16 antibody, CD-16, CD-32, CD16/32, CD16, CD-32 antibody, CD16/32 antibody (APC), FCGR3 antibody, CD 16 antibody, CD-16 antibody, CD32 antibody 16, CD32, CD 32 antibody, CD 16/32, FC Receptor Block antibody, FCGR2 antibody |
|---|---|
| Specificity | Mouse |
| Applications | FC, IHC, IP, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD16/32 antibody (allophycocyanin) (61R-1710)
Staining of BALB/c splenocytes with staining buffer (Autofluorescence) (open histogram) or 0.125 ug of CD16/CD32 antibody (filled histogram). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | 93 |
| Isotype | IgG2a lambda |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 2 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 and CD32 are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils. |
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