CD16/32 antibody (FITC) (61R-1058)
Rat monoclonal CD16/32 antibody (FITC)
Overview
Overview
| Synonyms | Monoclonal CD16/32 antibody, Anti-CD16/32 antibody, FC Receptor Block antibody, CD-16, CD 16/32, CD-32, CD 32 antibody, FCGR3 antibody, CD32 antibody 16, CD-32 antibody, CD-16 antibody, CD16, CD16/32, FCGR2 antibody, IGFR2, IGFR3, CD32, CD16 antibody, CD 16 antibody |
|---|---|
| Specificity | Mouse |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. |
Images
Flow Cytometric analysis using CD16/32 antibody (FITC) (61R-1058)
Staining of BALB/c splenocytes with CD45R antibody (PE) and 0.25 ug of CD16/CD32 antibody (FITC). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | 93 |
| Isotype | IgG2a lambda |
| Tag/Conjugate | FITC |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 5 ul (1 ug)/test |
Usage & Assay Information
| Usage Recommendations | FC: 5 ul (1 ug)/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 and CD32 are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils. |
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