CD16 antibody (10R-6359)
Mouse monoclonal CD16 antibody
Overview
Overview
| Synonyms | Monoclonal CD16 antibody, Anti-CD16 antibody, CD16, CD-16, CD 16 antibody, FCGR3 antibody, CD-16 antibody, FCGR3a antibody, IGFR3 antibody, CD 16, Low Affinity IgG Receptor 3 antibody |
|---|---|
| Specificity | Human |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Flow Cytometric analysis using CD16 antibody (10R-6359)
Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control (open histogram) or 0.25 ug of CD16 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | CB16 |
| Isotype | IgG1 |
| Method of Purification | CD16 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.125 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The CB16 monoclonal antibody recognizes CD16, the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A, which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B, which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity, phagocytosis, degranulation and proliferation. |
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