CD19 antibody (10R-6362)

Rat monoclonal CD19 antibody

Synonyms Monoclonal CD19 antibody, Anti-CD19 antibody, CD-19, B4 antibody, CD-19 antibody, Leu-12 antibody, CD19, CD 19 antibody, CD 19
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using CD19 antibody (10R-6362)

Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgG2a Isotype Control (open histogram) or 0.25 ug of CD19 antibody) (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.

Specifications

Host Rat
Clone 1D3
Isotype IgG2a kappa
Method of Purification CD19 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

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Images

  • Flow Cytometric analysis using CD19 antibody (10R-6362) | Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgG2a Isotype Control (open histogram) or 0.25 ug of CD19 antibody) (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.

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Price From: Price: €95.71

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Price From: Price: €95.71
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