CD19 antibody (10R-6362)
Rat monoclonal CD19 antibody
Overview
Overview
| Synonyms | Monoclonal CD19 antibody, Anti-CD19 antibody, CD-19, B4 antibody, CD-19 antibody, Leu-12 antibody, CD19, CD 19 antibody, CD 19 |
|---|---|
| Specificity | Mouse |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Flow Cytometric analysis using CD19 antibody (10R-6362)
Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgG2a Isotype Control (open histogram) or 0.25 ug of CD19 antibody) (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | 1D3 |
| Isotype | IgG2a kappa |
| Method of Purification | CD19 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. |
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