CD19 antibody (allophycocyanin) (61R-1711)

Mouse monoclonal CD19 antibody (allophycocyanin)

Synonyms Monoclonal CD19 antibody, Anti-CD19 antibody, CD19, CD-19, CD 19, CD19 antibody (APC), CD-19 antibody, AW495831 antibody, B4 antibody, CD 19 antibody
Specificity Mouse
Applications FC, Functional Assay, IHC, IP
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD19 antibody (allophycocyanin) (61R-1711)

Staining of mouse splenocytes with CD3e antibody (FITC) and staining buffer (Autofluorescence) (left) or 0.25 ug of CD19 antibody (right). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone MB19-1
Isotype IgA kappa
Tag/Conjugate Allophycocyanin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

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Images

  • Flow Cytometric analysis using CD19 antibody (allophycocyanin) (61R-1711) | Staining of mouse splenocytes with CD3e antibody (FITC) and staining buffer (Autofluorescence) (left) or 0.25 ug of CD19 antibody (right). Total viable cells were used for analysis.

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Price From: Price: €204.50

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Price From: Price: €204.50
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