CD19 antibody (allophycocyanin) (61R-1711)
Mouse monoclonal CD19 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD19 antibody, Anti-CD19 antibody, CD19, CD-19, CD 19, CD19 antibody (APC), CD-19 antibody, AW495831 antibody, B4 antibody, CD 19 antibody |
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| Specificity | Mouse |
| Applications | FC, Functional Assay, IHC, IP |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD19 antibody (allophycocyanin) (61R-1711)
Staining of mouse splenocytes with CD3e antibody (FITC) and staining buffer (Autofluorescence) (left) or 0.25 ug of CD19 antibody (right). Total viable cells were used for analysis.
Specifications
| Host | Mouse |
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| Clone | MB19-1 |
| Isotype | IgA kappa |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5. |
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