CD23 antibody (10R-6366)

Mouse monoclonal CD23 antibody

Synonyms Monoclonal CD23 antibody, Anti-CD23 antibody, CD-23 antibody, FceRII antibody, CD 23 antibody, IGEBF antibody, CD 23, Low Affinity IgE Receptor antibody, FcER2 antibody, CD-23, CD23
Specificity Human
Applications FC, Functional Assay, IHC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using CD23 antibody (10R-6366)

Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control (open histogram) or 0.25 ug of CD23 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.

Specifications

Host Mouse
Clone EBVCS2
Isotype IgG1
Method of Purification CD23 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23.

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Images

  • Flow Cytometric analysis using CD23 antibody (10R-6366) | Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control (open histogram) or 0.25 ug of CD23 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Cells in the lymphocyte gate were used for analysis.

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Price From: Price: €95.71
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