CD24 antibody (10R-6368)
Rat monoclonal CD24 antibody
Overview
Overview
| Synonyms | Monoclonal CD24 antibody, Anti-CD24 antibody, Heat Stable Antigen antibody, CD 24 antibody, CD 24, Ly-52 antibody, CD24, CD-24 antibody, CD-24, Nectadrin antibody, HSA antibody |
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| Specificity | Mouse |
| Applications | FC, IHC, IP, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD24 antibody (10R-6368)
Staining of BALB/c splenocytes with 0.06 ug of Rat IgG2b Isotype Control (open histogram) or 0.06 ug of CD24 antibody (filled histogram) followed by Anti-Rat IgG (FITC). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | M1/69 |
| Isotype | IgG2b kappa |
| Method of Purification | CD24 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen. This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin binds to CD24. |
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