CD25 antibody (10R-6370)
Mouse monoclonal CD25 antibody
Overview
Overview
| Synonyms | Monoclonal CD25 antibody, Anti-CD25 antibody, Tac antibody, CD 25 antibody, IL-2Ra antibody, IL2Ra antibody, CD25, CD 25, CD-25, CD-25 antibody, Interleukin-2 Receptor alpha antibody |
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| Specificity | Human |
| Applications | FC, Functional Assay, IHC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD25 antibody (10R-6370)
Staining of PHA stimulated human PBMC with CD25 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | BC96 |
| Isotype | IgG1 kappa |
| Method of Purification | CD25 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain. CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 and CD132 to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. |
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