CD25 antibody (10R-6370)

Mouse monoclonal CD25 antibody

Synonyms Monoclonal CD25 antibody, Anti-CD25 antibody, Tac antibody, CD 25 antibody, IL-2Ra antibody, IL2Ra antibody, CD25, CD 25, CD-25, CD-25 antibody, Interleukin-2 Receptor alpha antibody
Specificity Human
Applications FC, Functional Assay, IHC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.

Images

Flow Cytometric analysis using CD25 antibody (10R-6370)

Staining of PHA stimulated human PBMC with CD25 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone BC96
Isotype IgG1 kappa
Method of Purification CD25 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain. CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 and CD132 to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.

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Images

  • Flow Cytometric analysis using CD25 antibody (10R-6370) | Staining of PHA stimulated human PBMC with CD25 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Availability: In stock

Price: €149.69

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Price: €149.69
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