CD25 antibody (allophycocyanin) (61R-1715)
Mouse monoclonal CD25 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD25 antibody, Anti-CD25 antibody, CD 25, Interleukin-2 Receptor alpha antibody, CD 25 antibody, CD25, Tac antibody, IL2Ra antibody, CD-25, CD25 antibody (APC), CD-25 antibody, IL-2Ra antibody |
|---|---|
| Specificity | Human |
| Applications | FC, IP, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD25 antibody (allophycocyanin) (61R-1715)
Staining of normal human peripheral blood cells with CD4 antibody (PE) and Mouse IgG1 kappa Isotype Control (left) or CD25 antibody (right). Cells in the lymphocyte gate were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | BC96 |
| Isotype | IgG1 kappa |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain. CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 and CD132 to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. |
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