CD27 antibody (10R-6373)
Armenian Hamster monoclonal CD27 antibody
Overview
Overview
| Synonyms | Monoclonal CD27 antibody, Anti-CD27 antibody, CD27, CD 27, TNFRSF7 antibody, CD-27, T14 antibody, CD 27 antibody, CD-27 antibody |
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| Specificity | Mouse |
| Cross Reactivity | Human, Rat |
| Applications | FC, IHC, IP |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD27 antibody (10R-6373)
Staining of BALB/c splenocytes with CD45R antibody (FITC) and 0.125 ug CD27 antibody followed by Anti-Armenian Hamster IgG (PE). Total viable cells were analyzed.
Specifications
| Host | Hamster |
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| Clone | LG.3A10 |
| Isotype | IgG |
| Method of Purification | CD27 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.125 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The LG.3A10 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.8F10 cross-reacts with human and rat CD27.When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction. |
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