CD27 antibody (10R-6374)
Mouse monoclonal CD27 antibody
Overview
Overview
| Synonyms | Monoclonal CD27 antibody, Anti-CD27 antibody, CD-27, CD 27, TNFRSF7 antibody, CD 27 antibody, CD27, CD-27 antibody |
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| Specificity | Human |
| Applications | FC, Functional Assay, IHC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Flow Cytometric analysis using CD27 antibody (10R-6374)
Staining of normal human peripheral blood cells with CD27 antibody (PE) (left) and (right). Appropriate Isotype Controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | O323 |
| Isotype | IgG1 kappa |
| Method of Purification | CD27 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. |
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