CD28 antibody (allophycocyanin) (61R-1717)
Syrian Hamster monoclonal CD28 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD28 antibody, Anti-CD28 antibody, CD-28, CD 28 antibody, CD28, CD 28, Tp44 antibody, CD28 antibody (APC), CD-28 antibody |
|---|---|
| Specificity | Mouse |
| Applications | FC, IHC, IP |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD28 antibody (allophycocyanin) (61R-1717)
Staining of BALB/c splenocytes with CD3e antibody (FITC) and 0.25 ug of Golden Syrian Hamster IgG Isotype Control (left) or 0.25 ug of CD28 antibody (right). Total viable cells were used for analysis.
Specifications
| Host | Hamster |
|---|---|
| Clone | 37.51 |
| Isotype | IgG |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 and CD86 and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. |
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