CD28 antibody (allophycocyanin) (61R-1717)

Syrian Hamster monoclonal CD28 antibody (allophycocyanin)

Synonyms Monoclonal CD28 antibody, Anti-CD28 antibody, CD-28, CD 28 antibody, CD28, CD 28, Tp44 antibody, CD28 antibody (APC), CD-28 antibody
Specificity Mouse
Applications FC, IHC, IP
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD28 antibody (allophycocyanin) (61R-1717)

Staining of BALB/c splenocytes with CD3e antibody (FITC) and 0.25 ug of Golden Syrian Hamster IgG Isotype Control (left) or 0.25 ug of CD28 antibody (right). Total viable cells were used for analysis.

Specifications

Host Hamster
Clone 37.51
Isotype IgG
Tag/Conjugate Allophycocyanin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells and NK cells. CD28 is a ligand for CD80 and CD86 and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

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Images

  • Flow Cytometric analysis using CD28 antibody (allophycocyanin) (61R-1717) | Staining of BALB/c splenocytes with CD3e antibody (FITC) and 0.25 ug of Golden Syrian Hamster IgG Isotype Control (left) or 0.25 ug of CD28 antibody (right). Total viable cells were used for analysis.

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Price From: Price: €262.58

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Price From: Price: €262.58
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