CD29 antibody (10R-6379)
Mouse monoclonal CD29 antibody
Overview
Overview
| Synonyms | Monoclonal CD29 antibody, Anti-CD29 antibody, CD-29 antibody, Integrin 1 antibody, CD29, platelet GPIIa antibody, ITGB1 antibody, CD 29 antibody, CD-29, CD 29 |
|---|---|
| Specificity | Human |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Flow Cytometric analysis using CD29 antibody (10R-6379)
Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control (blue histogram) or 0.125 ug of CD29 antibody (purple histogram) followed by F (ab'')2 Anti-Mouse IgG (PE). Cells in the lymphocyte gate were used for analysis.
Specifications
| Host | Mouse |
|---|---|
| Clone | TS2/16 |
| Isotype | IgG1 |
| Method of Purification | CD29 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta1 integrins. |
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