CD29 antibody (allophycocyanin) (61R-1718)
Armenian Hamster monoclonal CD29 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD29 antibody, Anti-CD29 antibody, Integrin beta 1 antibody, ITGB1 antibody, CD29 antibody (APC), CD 29, platelet GPIIa antibody, CD-29, CD 29 antibody, CD29, CD-29 antibody |
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| Specificity | Mouse, Rat |
| Applications | IHC, WB |
| Assay Information | This 50 antibody has been tested by western blotting under reducing conditions (at 5 ug/ml) on Jurkat cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD29 antibody (allophycocyanin) (61R-1718)
Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (left) or 0.125 ug of CD29 antibody (right). Total viable cells were used for analysis.
Specifications
| Host | Hamster |
|---|---|
| Clone | HMb1-1 |
| Isotype | IgG |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | IHC: = 10 ug/ml |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The HMb1-1 monoclonal antibody reacts with mouse and rat CD29, a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alphabeta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1. |
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