CD3 antibody (FITC) (61R-1034)

Mouse monoclonal CD3 antibody (FITC)

Synonyms Monoclonal CD3 antibody, Anti-CD3 antibody, CD3, CD-3 antibody, CD 3 antibody, Leu-4 antibody, CD 3, CD-3, T3 antibody
Specificity Human
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD3 antibody (FITC) (61R-1034)

Staining of normal human peripheral blood cells with CD19 antibody (PE) (y-axis, left) or CD150 antibody (PE) (y-axis, right) and CD3 antibody (FITC) (x-axis).

Specifications

Host Mouse
Clone UCHT1
Isotype IgG1 kappa
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

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Images

  • Flow Cytometric analysis using CD3 antibody (FITC) (61R-1034) | Staining of normal human peripheral blood cells with CD19 antibody (PE) (y-axis, left) or CD150 antibody (PE) (y-axis, right) and CD3 antibody (FITC) (x-axis).

Availability: In stock

Price: €143.15
Size: 25 ug
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