CD3 antibody (FITC) (61R-1034)
Mouse monoclonal CD3 antibody (FITC)
Overview
Overview
| Synonyms | Monoclonal CD3 antibody, Anti-CD3 antibody, CD3, CD-3 antibody, CD 3 antibody, Leu-4 antibody, CD 3, CD-3, T3 antibody |
|---|---|
| Specificity | Human |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD3 antibody (FITC) (61R-1034)
Staining of normal human peripheral blood cells with CD19 antibody (PE) (y-axis, left) or CD150 antibody (PE) (y-axis, right) and CD3 antibody (FITC) (x-axis).
Specifications
| Host | Mouse |
|---|---|
| Clone | UCHT1 |
| Isotype | IgG1 kappa |
| Tag/Conjugate | FITC |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. |
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