CD31 antibody (biotin) (61R-CD31KBT)

Mouse monoclonal CD31 antibody (biotin)

Synonyms Monoclonal CD31 antibody, Anti-CD31 antibody, CD-31, CD 31 antibody, PECAM1 antibody, CD31, CD 31, PECAM-1 antibody, CD-31 antibody
Specificity Rat
Cross Reactivity To be determined by end-user
Applications FC
Immunogen CD31 antibody (Biotin) was raised in mouse using activated lewis rat derived microglial cells as the immunogen.

Images

Western blot analysis using CD31 antibody (biotin) (61R-CD31KBT)

Western blot analysis of cortical microvessel membrane- and cytosolic-enriched fractions probed for nPKC-theta and aPKC-zeta isozymes and phosphorylation state, (A) nPKC-theta (67kDa) showed increased protein expression in membrane fractions after hypoxia (Hx) treatment (30 or 60minutes). Hypoxia and reoxygenation (HR) (15minutes) treatment showed similar levels of expression as Hx (30minutes). Cytosolic fractions showed little change in protein expression under Hx (30 or 60minutes) and HR conditions. pPKC-theta (Thr538) (74kDa) was only detected in the membrane-enriched fraction and showed marked increased expression after Hx (30 or 60minutes). Expression was still elevated after HR. (B) aPKC-zeta showed increased protein expression during Hx treatment, which decreased to levels comparable to that detected in normoxic (Nx) tissue after HR. aPKC-zeta in cytosolic-enriched fractions showed no significant changes in expre

Specifications

Host Mouse
Clone M1081101
Isotype IgG1
Method of Purification CD31 antibody (biotin) was purified by Protein G affinity chromatography.
Tag/Conjugate Biotin
Form & Buffer PBS with 0.09% NaN3, and 0.1% BSA.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations FC: neat

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance Human CD31 is an adhesion molecule expressed on platelets, endothelial cells, leukocytes and their bone marrow precursors. CD31 plays a role in homophilic adhesion and heterophilic transendothelial migration.

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Images

  • Western blot analysis using CD31 antibody (biotin) (61R-CD31KBT) | Western blot analysis of cortical microvessel membrane- and cytosolic-enriched fractions probed for nPKC-theta and aPKC-zeta isozymes and phosphorylation state, (A) nPKC-theta (67kDa) showed increased protein expression in membrane fractions after hypoxia (Hx) treatment (30 or 60minutes). Hypoxia and reoxygenation (HR) (15minutes) treatment showed similar levels of expression as Hx (30minutes). Cytosolic fractions showed little change in protein expression under Hx (30 or 60minutes) and HR conditions. pPKC-theta (Thr538) (74kDa) was only detected in the membrane-enriched fraction and showed marked increased expression after Hx (30 or 60minutes). Expression was still elevated after HR. (B) aPKC-zeta showed increased protein expression during Hx treatment, which decreased to levels comparable to that detected in normoxic (Nx) tissue after HR. aPKC-zeta in cytosolic-enriched fractions showed no significant changes in expre
  • Immunohistochemical staining using CD31 antibody (biotin) (61R-CD31KBT) | Double label immunohistochemistry results from hepatocyte (A), Kupffer cell (B) and endothelial cell (C) isolates, respectively. After isolation, cells were applied to slides using a cytocentrifuge and incubated with anti-rat PECAM-1 (brown) and antirat mononuclear phagocyte (red) antibodies to identify endothelial and Kupffer cells, respectively. In the hepatocyte isolate, two Kupffer cells can be observed in the center of the field. Cells were visualized at 380 × magnification.

Availability: In stock

Price: €277.55
Size: 100 ug
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