CD4 antibody (allophycocyanin) (61R-1699)

Rat monoclonal CD4 antibody (allophycocyanin)

Synonyms Monoclonal CD4 antibody, Anti-CD4 antibody, CD 4, CD 4 antibody, CD4, CD-4 antibody, CD4 antibody (APC), CD-4, L3T4 antibody, Ly-4 antibody
Specificity Mouse
Applications FC, WB
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD4 antibody (allophycocyanin) (61R-1699)

Staining of C57Bl/6 splenocytes with CD45R antibody (FITC) and 0.06 ug of Rat IgG2b K Isotype Control (left) or 0.06 ug of CD4 antibody (right). Total viable cells were used for analysis.

Specifications

Host Rat
Clone GK1.5
Isotype IgG2b kappa
Tag/Conjugate Allophycocyanin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1.0 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

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Images

  • Flow Cytometric analysis using CD4 antibody (allophycocyanin) (61R-1699) | Staining of C57Bl/6 splenocytes with CD45R antibody (FITC) and 0.06 ug of Rat IgG2b K Isotype Control (left) or 0.06 ug of CD4 antibody (right). Total viable cells were used for analysis.

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Price From: Price: €192.23

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Price From: Price: €192.23
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