CD4 antibody (allophycocyanin) (61R-1700)
Rat monoclonal CD4 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD4 antibody, Anti-CD4 antibody, CD4, CD-4 antibody, CD 4 antibody, Ly-4 antibody, CD-4, CD4 antibody (APC), CD 4, L3T4 antibody |
|---|---|
| Specificity | Mouse |
| Applications | FC, IHC, IP, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD4 antibody (allophycocyanin) (61R-1700)
Staining of mouse splenocytes with CD3 antibody (FITC) and 0.06 ug of Rat IgG2a K Isotype Control (left) or 0.06 ug of CD4 antibody (right). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | RM4-5 |
| Isotype | IgG2a kappa |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5. |
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