CD4 antibody (FITC) (61R-1037)

Rat monoclonal CD4 antibody (FITC)

Synonyms Monoclonal CD4 antibody, Anti-CD4 antibody, CD 4, CD4, Ly-4 antibody, CD 4 antibody, L3T4 antibody, CD-4, CD-4 antibody
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest

Images

Flow Cytometric analysis using CD4 antibody (FITC) (61R-1037)

Staining of C57BL/6 splenocytes with CD3e antibody (PE) and 0.125 ug of Rat IgG2a K Isotype Control (FITC) (left) or 0.125 ug of CD4 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.

Specifications

Host Rat
Clone RM4-5
Isotype IgG2a kappa
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

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Images

  • Flow Cytometric analysis using CD4 antibody (FITC) (61R-1037) | Staining of C57BL/6 splenocytes with CD3e antibody (PE) and 0.125 ug of Rat IgG2a K Isotype Control (FITC) (left) or 0.125 ug of CD4 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.

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Price From: Price: €95.71

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Price From: Price: €95.71
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