CD4 antibody (FITC) (61R-1038)

Rat monoclonal CD4 antibody (FITC)

Synonyms Monoclonal CD4 antibody, Anti-CD4 antibody, CD4, CD 4 antibody, CD 4, CD-4 antibody, Ly-4 antibody, CD-4, L3T4 antibody
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD4 antibody (FITC) (61R-1038)

Staining of C57BL/6 splenocytes with 0.125 ug of Rat IgG2b kappa Isotype Control (FITC) (open histogram) or 0.06 ug of CD4 antibody (FITC) (filled histogram). Cells in the lymphocyte gate were used for analysis.

Specifications

Host Rat
Clone RM4-4
Isotype IgG2b kappa
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.125 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of the CD4 monoclonal antibodies RM4-5 or GK1.5.

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Images

  • Flow Cytometric analysis using CD4 antibody (FITC) (61R-1038) | Staining of C57BL/6 splenocytes with 0.125 ug of Rat IgG2b kappa Isotype Control (FITC) (open histogram) or 0.06 ug of CD4 antibody (FITC) (filled histogram). Cells in the lymphocyte gate were used for analysis.

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Price From: Price: €125.97

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Price From: Price: €125.97
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