CD40 antibody (10R-6391)
Armenian Hamster monoclonal CD40 antibody
Overview
Overview
| Synonyms | Monoclonal CD40 antibody, Anti-CD40 antibody, TNFRSF5 antibody, CD 40, CD-40, CD-40 antibody, CD 40 antibody, CD40 |
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| Specificity | Mouse, Rat |
| Applications | FC, Functional Assay, IHC, IP |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Flow Cytometric analysis using CD40 antibody (10R-6391)
Staining of BALB/c splenocytes with 0.5 ug Armenian Hamster IgG Isotype Control (open histogram) or 0.5 ug CD40 antibody (filled histogram) followed by Anti-Armenian Hamster IgG (FITC). Total viable cells were used for analysis.
Specifications
| Host | Hamster |
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| Clone | HM40-3 |
| Isotype | IgM kappa |
| Method of Purification | CD40 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.25 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. |
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