CD43 antibody (10R-6397)

Rat monoclonal CD43 antibody

Synonyms Monoclonal CD43 antibody, Anti-CD43 antibody, Leukosialin antibody, CD-43, SPN antibody, Sialophorin antibody, CD-43 antibody, CD 43, LSN antibody, Ly-48 antibody, CD 43 antibody, CD43
Specificity Mouse
Applications FC, IHC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using CD43 antibody (10R-6397)

Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control (open histogram) or 0.25 ug of CD43 antibody (filled histogram) followed by Anti-Rat IgM (PE). Cells in the lymphocyte gate were used for analysis.

Specifications

Host Rat
Clone R2/60
Isotype IgM
Method of Purification CD43 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Flow Cytometric analysis using CD43 antibody (10R-6397) | Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control (open histogram) or 0.25 ug of CD43 antibody (filled histogram) followed by Anti-Rat IgM (PE). Cells in the lymphocyte gate were used for analysis.

Availability: In stock

Price From: Price: €77.71

* Required Fields

Price From: Price: €77.71
Shipping
View Our Distributors