CD43 antibody (10R-6397)
Rat monoclonal CD43 antibody
Overview
Overview
| Synonyms | Monoclonal CD43 antibody, Anti-CD43 antibody, Leukosialin antibody, CD-43, SPN antibody, Sialophorin antibody, CD-43 antibody, CD 43, LSN antibody, Ly-48 antibody, CD 43 antibody, CD43 |
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| Specificity | Mouse |
| Applications | FC, IHC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
Images
Flow Cytometric analysis using CD43 antibody (10R-6397)
Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control (open histogram) or 0.25 ug of CD43 antibody (filled histogram) followed by Anti-Rat IgM (PE). Cells in the lymphocyte gate were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | R2/60 |
| Isotype | IgM |
| Method of Purification | CD43 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43. |
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