A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. The HI98 antibody has also been tested by immunohistochemistry on FFPE tisue (IHC-P) at less than or equal to 10 ug/ml. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Store at 2-8 deg C. Do not freeze. Protect from light.
General Information
Biological Significance
The monoclonal antibody 84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.