CD44 antibody (10R-1087)
Mouse monoclonal CD44 antibody
Overview
Overview
| Synonyms | Monoclonal CD44 antibody, Anti-CD44 antibody, CD44, CD44 antigen (homing function and Indian blood group system) antibody, CD 44, chondroitin sulfate proteoglycan 8 antibody, MDU2 antibody, CSPG8 antibody, IN antibody, CD-44 antibody, CD44R antibody, MDU3 antibody, hematopoietic cell E- and L-selectin ligand antibody, CD44 molecule (Indian blood group) antibody, HCELL antibody, CD 44 antibody, MIC4 antibody, CD-44, CD44 antigen isoform 3 precursor antibody, MC56 antibody, Pgp1 antibody |
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| Specificity | Human |
| Cross Reactivity | To be determined by end-user. |
| Applications | ELISA, WB |
| Immunogen | CD44 antibody was raised in mouse using recombinant human CD44 (21-145aa) purified from E. coli as the immunogen. |
Images
Western Blot analysis of CD44 antibody (10R-1087)
The Cell lysates of U937 (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human CD44 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Specifications
| Host | Mouse |
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| Clone | 5C10 |
| Isotype | IgG2b kappa |
| Source | Anti-human CD44 antibody, clone 5C10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD44 protein. |
| Method of Purification | CD44 antibody was purified by protein-G affinity chromatography |
| Form & Buffer | Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:1,000-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | CD44 is a broadly distributed transmembrane glycoprotein that plays a critical role in a variety of cellular behaviors, including adhesion, migration, invasion, and survival. CD44 mediates cell–cell and cell–matrix interactions in a large part through its affinity for hyaluronan (HA), a glycosaminoglycan constituent of extracellular matrices, but also potentially through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Variants of CD44 are expressed in tissues during development, including embryonic epithelia. known functions of CD44 are cellular adhesion (aggregation and migration), hyaluronate degradation, lymphocyte activation, lymph node homing, myelopoiesis and lymphopoiesis, angiogenesis, and release of cytokines. The functions of CD44 are principally dependant on cellular adhesion in one setting or another |
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