CD45 antibody (10R-6402)
Rat monoclonal CD45 antibody
Overview
Overview
| Synonyms | Monoclonal CD45 antibody, Anti-CD45 antibody, CD 45, CD45, CD 45 antibody, CD-45, Ly-5 antibody, Leukocyte Common Antigen antibody, CD-45 antibody, LCA antibody, SCID due to PTPRC deficiency antibody, LCA antibody, Protein tyrosine phosphatase receptor type c polypeptide antibody, B220 antibody, receptor type antibody, T200 glycoprotein antibody, protein tyrosine phosphatase antibody, L CA antibody, Leukocyte common antigen antibody |
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| Specificity | Mouse |
| Applications | FC, Functional Assay, IHC, IP, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD45 antibody (10R-6402)
Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgG2b Isotype Control (open histogram) or 0.25 ug of CD45 antibody (filled histogram) followed by Anti-Rat IgG (FITC). Cells in the lymphocyte gate were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | 30-F11 |
| Isotype | IgG2b kappa |
| Method of Purification | CD45 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen. CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. |
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