CD45 antibody (allophycocyanin) (61R-1730)
Rat monoclonal CD45 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD45 antibody, Anti-CD45 antibody, CD-45, LCA antibody, Leukocyte Common Antigen antibody, CD 45 antibody, CD 45, CD45, Ly-5 antibody, CD45 antibody (APC), CD-45 antibody |
|---|---|
| Specificity | Mouse |
| Applications | FC, Functional Assay |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD45 antibody (allophycocyanin) (61R-1730)
Staining of C57BL/6 splenocytes with staining buffer (Autofluorescence) (open histogram) or 0.06 ug of CD45 antibody (filled histogram). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | 30-F11 |
| Isotype | IgG2b kappa |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen. CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. |
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