Flow cytometric assay using CD49d antibody (10R-CD49daHU)
Effector T cells primed by dendritic cells (DC) from mesenteric (mLN) and axillary (axLN) lymph nodes differ in their cytokine release. Surface expression was measured on mLN- and axLN-primed effector T cells after culture and before injection into recipients. (a) The percentage of various markers among CD4+ primed T cells. Means and SEM (n = 5 or n = 6) are given. Asterisks indicate significant differences between surface expression of mLN- and axLN-primed CD4+ T cells in the Student’s t-test (*P < 0·05). The mLN-primed CD4+ T cells showed an activated phenotype. (b) The same analysis was performed for mLN- and axLN-primed CD8+ T cells. No differences were seen between the two populations. (c) Supernatants of the cultured T cells with DC from mLN and axLN were collected and analysed by enzyme-linked immunosorbent assay (ELISA). Whereas interferon-γ (IFN-γ) was produced more among the mLN-primed T cells after MAM stimulation,
CD49d antibody
Flow cytometric assay using CD49d antibody (10R-CD49daHU)
Effector T cells primed by dendritic cells (DC) from mesenteric (mLN) and axillary (axLN) lymph nodes differ in their cytokine release. Surface expression was measured on mLN- and axLN-primed effector T cells after culture and before injection into recipients. (a) The percentage of various markers among CD4+ primed T cells. Means and SEM (n = 5 or n = 6) are given. Asterisks indicate significant differences between surface expression of mLN- and axLN-primed CD4+ T cells in the Student’s t-test (*P < 0·05). The mLN-primed CD4+ T cells showed an activated phenotype. (b) The same analysis was performed for mLN- and axLN-primed CD8+ T cells. No differences were seen between the two populations. (c) Supernatants of the cultured T cells with DC from mLN and axLN were collected and analysed by enzyme-linked immunosorbent assay (ELISA). Whereas interferon-γ (IFN-γ) was produced more among the mLN-primed T cells after MAM stimulation,
Specifications
Host
Mouse
Clone
HP2/1
Isotype
IgG1
Method of Purification
CD49d antibody was purified by Protein G affinity chromatography.
Form & Buffer
Supplied as a liquid in borate buffed saling, pH 8.8, with 0.09% NaN3.
Concentration
Batch dependent - please inquire should you have specific requirements
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
General Information
Biological Significance
Integrin alpha 4 is also known as CD49D. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.