CD49d antibody (10R-CD49daHU)

Mouse monoclonal CD49d antibody

Synonyms Monoclonal CD49d antibody, Anti-CD49d antibody, CD 49d antibody, CD-49d antibody, CD49d, CD 49d, CD-49d, VLA4a antibody
Specificity Human
Cross Reactivity Bovine, goat, horse, llama, mink, mustelid, cynomolgus monkey, pig, rabbit, rat, rhesus monkey
Applications FC, IHC-F, IP
Immunogen CD49d antibody was raised in mouse using JM leukaemia line as the immunogen.

Images

Flow cytometric assay using CD49d antibody (10R-CD49daHU)

Effector T cells primed by dendritic cells (DC) from mesenteric (mLN) and axillary (axLN) lymph nodes differ in their cytokine release. Surface expression was measured on mLN- and axLN-primed effector T cells after culture and before injection into recipients. (a) The percentage of various markers among CD4+ primed T cells. Means and SEM (n = 5 or n = 6) are given. Asterisks indicate significant differences between surface expression of mLN- and axLN-primed CD4+ T cells in the Student’s t-test (*P < 0·05). The mLN-primed CD4+ T cells showed an activated phenotype. (b) The same analysis was performed for mLN- and axLN-primed CD8+ T cells. No differences were seen between the two populations. (c) Supernatants of the cultured T cells with DC from mLN and axLN were collected and analysed by enzyme-linked immunosorbent assay (ELISA). Whereas interferon-γ (IFN-γ) was produced more among the mLN-primed T cells after MAM stimulation,

Specifications

Host Mouse
Clone HP2/1
Isotype IgG1
Method of Purification CD49d antibody was purified by Protein G affinity chromatography.
Form & Buffer Supplied as a liquid in borate buffed saling, pH 8.8, with 0.09% NaN3.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations FC: 1 ug/5 x10^5 cells

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance Integrin alpha 4 is also known as CD49D. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.

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Images

  • Flow cytometric assay using CD49d antibody (10R-CD49daHU) | Effector T cells primed by dendritic cells (DC) from mesenteric (mLN) and axillary (axLN) lymph nodes differ in their cytokine release. Surface expression was measured on mLN- and axLN-primed effector T cells after culture and before injection into recipients. (a) The percentage of various markers among CD4+ primed T cells. Means and SEM (n = 5 or n = 6) are given. Asterisks indicate significant differences between surface expression of mLN- and axLN-primed CD4+ T cells in the Student’s t-test (*P < 0·05). The mLN-primed CD4+ T cells showed an activated phenotype. (b) The same analysis was performed for mLN- and axLN-primed CD8+ T cells. No differences were seen between the two populations. (c) Supernatants of the cultured T cells with DC from mLN and axLN were collected and analysed by enzyme-linked immunosorbent assay (ELISA). Whereas interferon-γ (IFN-γ) was produced more among the mLN-primed T cells after MAM stimulation,

Availability: In stock

Price: €465.72
Size: 200 ug
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