CD49e antibody (10R-6414)
Armenian Hamster monoclonal CD49e antibody
Overview
Overview
| Synonyms | Monoclonal CD49e antibody, Anti-CD49e antibody, CD-49e, ITGA5 antibody, CD49e, CD-49e antibody, CD 49e, VLA5A antibody, CD 49e antibody, Integrin a5 antibody |
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| Specificity | Mouse |
| Applications | FC, Functional Assay, IP |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD49e antibody (10R-6414)
Staining of C57Bl/6 bone marrow cells with 0.5 ug of Armenian Hamster IgG Isotype Control (left) or 0.5 ug of CD49e antibody (right) followed by Anti-Armenian Hamster IgG (FITC). Total viable cells were used for analysis.
Specifications
| Host | Hamster |
|---|---|
| Clone | HMa5-1 |
| Isotype | IgG |
| Method of Purification | CD49e antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The HM alpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The HM alpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HM alpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis. |
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