CD69 antibody (10R-6432)

Armenian Hamster monoclonal CD69 antibody

Synonyms Monoclonal CD69 antibody, Anti-CD69 antibody, CD 69 antibody, VEA antibody, CD 69, AIM antibody, CD69, CD-69, mlR3 antibody, CD-69 antibody
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.

Images

Flow Cytometric analysis using CD69 antibody (10R-6432)

Surface staining of ConA stimulated splenocytes with CD69 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Specifications

Host Hamster
Clone H1.2F3
Isotype IgG
Method of Purification CD69 antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen. CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

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Images

  • Flow Cytometric analysis using CD69 antibody (10R-6432) | Surface staining of ConA stimulated splenocytes with CD69 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

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Price From: Price: €104.70

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Price From: Price: €104.70
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