CD69 antibody (10R-6432)
Armenian Hamster monoclonal CD69 antibody
Overview
Overview
| Synonyms | Monoclonal CD69 antibody, Anti-CD69 antibody, CD 69 antibody, VEA antibody, CD 69, AIM antibody, CD69, CD-69, mlR3 antibody, CD-69 antibody |
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| Specificity | Mouse |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
Images
Flow Cytometric analysis using CD69 antibody (10R-6432)
Surface staining of ConA stimulated splenocytes with CD69 antibody (PE). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.
Specifications
| Host | Hamster |
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| Clone | H1.2F3 |
| Isotype | IgG |
| Method of Purification | CD69 antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen. CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation. |
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