CD71 antibody (allophycocyanin) (61R-1741)
Rat monoclonal CD71 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD71 antibody, Anti-CD71 antibody, CD-71, CD 71 antibody, TFRC antibody, TFR antibody, CD-71 antibody, TRFR antibody, CD71 antibody (APC), TFR1 antibody, CD 71, CD71 |
|---|---|
| Specificity | Mouse |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD71 antibody (allophycocyanin) (61R-1741)
Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.06 ug of Rat IgG2a K Isotype Control (left) or 0.06 ug of CD71 antibody (Transferrin Receptor) (right). Cells in the small scatter population were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | R17217 |
| Isotype | IgG2a kappa |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 200 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.125 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. |
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