CD8 antibody (allophycocyanin) (61R-1704)
Rat monoclonal CD8 antibody (allophycocyanin)
Overview
Overview
| Synonyms | Monoclonal CD8 antibody, Anti-CD8 antibody, CD 8 antibody, CD-8, Ly-35 antibody, CD8 alpha antibody, CD 8, Ly-2 antibody, Ly-B antibody, Lyt-2 antibody, CD8 antibody (APC), CD-8 antibody, CD8 |
|---|---|
| Specificity | Mouse |
| Applications | FC, WB |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD8 antibody (allophycocyanin) (61R-1704)
Staining of mouse splenocytes with CD3 antibody (FITC) and 0.06 ug of Rat IgG2a K Isotype Control (left) or 0.06 ug of Anti-Mouse CD8 (right). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | 53-6.7 |
| Isotype | IgG2a kappa |
| Tag/Conjugate | Allophycocyanin |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 1 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alphabeta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. |
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