CD8 antibody (allophycocyanin) (61R-1704)

Rat monoclonal CD8 antibody (allophycocyanin)

Synonyms Monoclonal CD8 antibody, Anti-CD8 antibody, CD 8 antibody, CD-8, Ly-35 antibody, CD8 alpha antibody, CD 8, Ly-2 antibody, Ly-B antibody, Lyt-2 antibody, CD8 antibody (APC), CD-8 antibody, CD8
Specificity Mouse
Applications FC, WB
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD8 antibody (allophycocyanin) (61R-1704)

Staining of mouse splenocytes with CD3 antibody (FITC) and 0.06 ug of Rat IgG2a K Isotype Control (left) or 0.06 ug of Anti-Mouse CD8 (right). Total viable cells were used for analysis.

Specifications

Host Rat
Clone 53-6.7
Isotype IgG2a kappa
Tag/Conjugate Allophycocyanin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alphabeta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

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Images

  • Flow Cytometric analysis using CD8 antibody (allophycocyanin) (61R-1704) | Staining of mouse splenocytes with CD3 antibody (FITC) and 0.06 ug of Rat IgG2a K Isotype Control (left) or 0.06 ug of Anti-Mouse CD8 (right). Total viable cells were used for analysis.

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Price From: Price: €251.12
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