CD8 antibody (allophycocyanin) (61R-1705)

Rat monoclonal CD8 antibody (allophycocyanin)

Synonyms Monoclonal CD8 antibody, Anti-CD8 antibody, CD 8, Ly-3 antibody, Lyt-3 antibody, CD-8, Ly-C antibody, CD8 beta antibody, CD-8 antibody, CD 8 antibody, CD8, CD8 antibody (APC)
Specificity Mouse
Applications FC, IHC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD8 antibody (allophycocyanin) (61R-1705)

Staining of C57Bl/6 splenocytes with CD8a antibody (FITC) and 0.25 ug of CD8b antibody (APC). Total viable cells were used for analysis.

Specifications

Host Rat
Clone H35-17.2
Isotype IgG2b kappa
Tag/Conjugate Allophycocyanin
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 1 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The H35-17.2 monoclonal antibody reacts with the mouse CD8beta molecule. The CD8beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

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Images

  • Flow Cytometric analysis using CD8 antibody (allophycocyanin) (61R-1705) | Staining of C57Bl/6 splenocytes with CD8a antibody (FITC) and 0.25 ug of CD8b antibody (APC). Total viable cells were used for analysis.

Availability: In stock

Price: €236.40
Size: 50 ug
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