CD80 antibody (FITC) (61R-1108)
Armenian Hamster monoclonal CD80 antibody (FITC)
Overview
Overview
| Synonyms | Monoclonal CD80 antibody, Anti-CD80 antibody, Ly-53 antibody, CD-80, CD 80 antibody, B7 antibody, CD-80 antibody, CD 80, CD80 |
|---|---|
| Specificity | Mouse |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Images
Flow Cytometric analysis using CD80 antibody (FITC) (61R-1108)
Staining of unstimulated (left) or 3-day LPS stimulated (right) C57BL/6 splenocytes with 0.125 ug of CD80 antibody (FITC). Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.
Specifications
| Host | Hamster |
|---|---|
| Clone | 16-10A1 |
| Isotype | IgG |
| Tag/Conjugate | FITC |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.5 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The 16-10A1 monoclonal antibody reacts with mouse CD80, a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152. The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. |
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