CD80 antibody (FITC) (61R-1108)

Armenian Hamster monoclonal CD80 antibody (FITC)

Synonyms Monoclonal CD80 antibody, Anti-CD80 antibody, Ly-53 antibody, CD-80, CD 80 antibody, B7 antibody, CD-80 antibody, CD 80, CD80
Specificity Mouse
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Images

Flow Cytometric analysis using CD80 antibody (FITC) (61R-1108)

Staining of unstimulated (left) or 3-day LPS stimulated (right) C57BL/6 splenocytes with 0.125 ug of CD80 antibody (FITC). Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.

Specifications

Host Hamster
Clone 16-10A1
Isotype IgG
Tag/Conjugate FITC
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.5 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The 16-10A1 monoclonal antibody reacts with mouse CD80, a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152. The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

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Images

  • Flow Cytometric analysis using CD80 antibody (FITC) (61R-1108) | Staining of unstimulated (left) or 3-day LPS stimulated (right) C57BL/6 splenocytes with 0.125 ug of CD80 antibody (FITC). Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.

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Price From: Price: €125.97

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Price From: Price: €125.97
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