CD93 antibody (FITC) (61R-1150)
Rat monoclonal CD93 antibody (FITC)
Overview
Overview
| Synonyms | Monoclonal CD93 antibody, Anti-CD93 antibody, CD-93, CD 93, CD-93 antibody, CD93, CD 93 antibody, C1qRp antibody, Early B lineage antigen antibody |
|---|---|
| Specificity | Mouse |
| Applications | FC, ICC, IHC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. The II/41 antibody has also been tested by immunocytochemistry on fixed and permeabilized cells and can be used at less than or equal to 2.5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest |
Images
Flow Cytometric analysis using CD93 antibody (FITC) (61R-1150)
Staining of C57BL/6 bone marrow cells with CD45R antibody (PE) and 0.5 ug of Rat IgG2b kappa Isotype Control (FITC) (left) or 0.5 ug of CD93 antibody (FITC) (right). Total viable cells were used for analysis.
Specifications
| Host | Rat |
|---|---|
| Clone | AA4.1 |
| Isotype | IgG2b kappa |
| Tag/Conjugate | FITC |
| Form & Buffer | Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.125 ug/test |
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Storage & Safety
| Storage | Store at 2-8 deg C. Do not freeze. Protect from light. |
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General Information
| Biological Significance | The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1 cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM, CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1, T2, and T3. AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule. |
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