CDC34 protein (His tag) (80R-1472)
Purified recombinant Human CDC34 protein
|Synonyms||UBCH3 protein, UBE2R1, CDC-34, CDC 34, CDC34, CDC 34 protein, CDC-34 protein, Ubiquitin-conjugating enzyme E2 R1 protein, E2-CDC34 protein, UBC3 protein|
Coomassie Blue stained SDS-PAGE of CDC34 protein (His tag) (80R-1472)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-236 amino acids: MGSSHHHHHH SSGLVPRGSH MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES|
|Grade & Purity||> 95% pure|
|Molecular Weight||28.9 kDa (256aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CDC34 is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. CDC34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae CDC34, which is essential for the G1 to S phase transition. Recombinant human CDC34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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