CDC42 protein (T7 tag) (80R-1200)
Purified recombinant Human CDC42 protein (T7 tag)
|Synonyms||CDC 42 protein, G25K protein, CDC-42 protein, CDC 42, Small GTP binding protein CDC42., Cell division cycle 42 protein, Cell division cycle 42 isoform 1 CDC42Hs protein, CDC42, CDC-42|
Coomassie Blue stained SDS-PAGE of CDC42 protein (T7 tag) (80R-1200)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-188 amino acids: MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC|
|Grade & Purity||> 95% pure|
|Molecular Weight||22.4 kDa (203aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol, and 2mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method )|
|Biological Significance||Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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