CDC45L antibody (70R-13279)

Affinity purified Rabbit polyclonal CDC45L antibody

Synonyms Polyclonal CDC45L antibody, Anti-CDC45L antibody, CDC45-related protein antibody, CDCL 45 antibody, CDCL-45 antibody, CDC45L antibody, PORC-PI-1 antibody, CDC45 cell division cycle 45 homolog antibody, cell division control protein 45 homolog antibody, CDC45 antibody, CDCL-45, human CDC45 antibody, CDCL 45, CDC45L2 antibody, cell division cycle 45 homolog (S. cerevisiae) antibody, cell division cycle 45-like 2 antibody, CDC45L
Cross Reactivity Human
Applications ICC, IF, WB

Images

Immunofluorescent staining using CDC45L antibody (70R-13279)

Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDC45L antibody at 1:200 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification CDC45L antibody was purified by antigen-affinity chromatography
Molecular Weight 66 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:200, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using CDC45L antibody (70R-13279)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed A549, using CDC45L antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using CDC45L antibody (70R-13279)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 7.5 % SDS PAGE gel and CDC45L antibody at a dilution of 1:1000

Availability: In stock

Price: €395.09
Size: 100 ul
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