CERT protein (His tag) (80R-1343)
Purified recombinant Human CERT protein
Overview
Overview
| Synonyms | Goodpasture protein binding protein protein, CERTL protein, STARD11 protein, FLJ20597 protein, START domain containing 11 protein, Ceramide transporter protein, Collagen type IV alpha 3 (Goodpasture protein) binding protein protein, CERT protein, alpha 3 binding protein Alpha 3 type IV collagen binding protein protein, , StARD 11 protein, START domain containing protein 11 protein, Procollagen type IV alpha 3 (Goodpasture protein) binding protein protein, StAR related lipid transfer protein 11 protein, Cert protein protein, Collagen protein, COL4A3BP protein, Lipid transfer protein CERTL protein, Collagen type IV alpha 3 binding protein protein, type IV protein, GPBP protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of CERT protein (His tag) (80R-1343)
Figure annotation denotes ug of protein loaded and % gel used.
CERT protein (His tag)
Coomassie Blue stained SDS-PAGE of CERT protein (His tag) (80R-1343)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 347-598 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMLH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 33.1 kDa (290aa) confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl. |
| Concentration | 0.5 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture protein. Goodpasture disease is the result of an autoimmune response directed at this protein. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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