CFTR antibody (10R-C123a)
Mouse monoclonal CFTR antibody
Overview
Overview
| Synonyms | Monoclonal CFTR antibody, Anti-CFTR antibody, Cystic Fibrosis Transmembrane Conductance Regulator antibody |
|---|---|
| Specificity | Human |
| Cross Reactivity | To be determined by end-user |
| Applications | ICC, IP, WB |
| Immunogen | CFTR antibody was raised in mouse using a synthetic peptide(RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR) as the immunogen. |
Images
Western blot analysis using CFTR antibody (10R-C123a)
Western Blot Analysis: T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- CFTR (0.5 ug/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates CFTR (~170 kDa)
Specifications
| Host | Mouse |
|---|---|
| Clone | MM13-4 |
| Isotype | IgG1 kappa |
| Method of Purification | CFTR antibody was purified by Protein A chromatography. |
| Molecular Weight | 170 kDa (predicted detection band MW) |
| Form & Buffer | Protein G purified antibody supplied in 100mM Tris-glycine, pH 7.4, with 0.15M NaCl, and 0.05% NaN3. |
| Concentration | Batch dependent - please inquire should you have specific requirements |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling. |
General Information
| Biological Significance | CFTR (Cystic Fibrosis Transmembrane conductance Regulator) is a 170 kDa chloride (Cl-) channel that regulates salt and water transport across epithelial tissues, where it modulates production of mucus, sweat, saliva, tears, and digestive enzymes. Cystic fibrosis patients have mutations in this protein, resulting in defective ion transport. |
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