ChAT antibody (70R-10625)

Affinity purified Chicken polyclonal ChAT antibody

Synonyms Polyclonal ChAT antibody, Anti-ChAT antibody, Choline Acetyltransferase antibody
Applications ICC, IHC
Immunogen ChAT antibody was raised in Chicken using a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate (Human P28329 and Mouse Q03059) as the immunogen.

Images

Immunofluorescent staining of ChAT antibody (70R-10625)

A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of green fluorescent protein (GFP) in this transgenic animal.

Specifications

Host Chicken
Method of Purification ChAT antibody was purified by affinity chromatography.
Form & Buffer Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 1:1000-1:2000; IHC: 1:1000-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light.

General Information

Biological Significance Choline acetyltransferase is an enzyme that is synthesized within the body of a neuron. It is then transferred to the nerve terminal via axoplasmic flow. The role of choline acetyltransferase is to join Acetyl-CoA to choline, resulting in the formation of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enyzme is encoded by the CHAT gene.

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Images

  • Immunofluorescent staining of ChAT antibody (70R-10625) | A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of green fluorescent protein (GFP) in this transgenic animal.

Availability: In stock

Price: €564.42
Size: 1 ml
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