ChAT antibody (70R-10625)
Affinity purified Chicken polyclonal ChAT antibody
Overview
Overview
| Synonyms | Polyclonal ChAT antibody, Anti-ChAT antibody, Choline Acetyltransferase antibody |
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| Applications | ICC, IHC |
| Immunogen | ChAT antibody was raised in Chicken using a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate (Human P28329 and Mouse Q03059) as the immunogen. |
Images
Immunofluorescent staining of ChAT antibody (70R-10625)
A tissue section through an adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of green fluorescent protein (GFP) in this transgenic animal.
Specifications
| Host | Chicken |
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| Method of Purification | ChAT antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative. |
| Concentration | 100 ug/ml |
Usage & Assay Information
| Usage Recommendations | ICC: 1:1000-1:2000; IHC: 1:1000-1:2000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Choline acetyltransferase is an enzyme that is synthesized within the body of a neuron. It is then transferred to the nerve terminal via axoplasmic flow. The role of choline acetyltransferase is to join Acetyl-CoA to choline, resulting in the formation of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enyzme is encoded by the CHAT gene. |
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