Chicken anti Rabbit IgG (H + L) (FITC) (43R-1435)
Chicken anti Rabbit IgG (H + L) secondary antibody (FITC)
Overview
Overview
| Synonyms | Chicken anti Rabbit Immunoglobulin G (H + L) secondary antibody (FITC) |
|---|---|
| Specificity | This antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins. |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin rabbit serum proteins or IgG from human or mouse. |
| Applications | FC, IF, IHC |
| Immunogen | Purified Rabbit IgG (H&L) |
Specifications
| Host | Chicken |
|---|---|
| Source | Ig fraction was prepared using eggs obtained from healthy hens of US origin. |
| Grade & Purity | > 95% pure |
| Method of Purification | Affinity purified using solid phase Rabbit IgG (H&L) |
| Tag/Conjugate | FITC |
| Form & Buffer | Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. |
| Concentration | 0.5 mg/ml |
Usage & Assay Information
| Usage Recommendations | The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications |
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Storage & Safety
| Storage | Store at 2-8 deg C. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
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