Chicken anti Rat IgG (H + L) (Alk Phos) (43R-1510)
Chicken anti Rat IgG (H + L) secondary antibody (Alk Phos)
Overview
Overview
| Synonyms | Chicken anti Rat Immunoglobulin G (H + L) secondary antibody (Alk Phos) |
|---|---|
| Specificity | This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins. |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or rabbit or serum proteins from human or rabbit. |
| Applications | User optimized |
| Immunogen | Purified Rat IgG (H&L) |
Specifications
| Host | Chicken |
|---|---|
| Source | Ig fraction was prepared using eggs obtained from healthy hens of US origin. |
| Grade & Purity | > 95% pure |
| Method of Purification | Affinity purified using solid phase Rat IgG (H&L) |
| Tag/Conjugate | Alkaline Phosphatase |
| Form & Buffer | Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. |
| Concentration | 1.5 mg/ml |
Usage & Assay Information
| Usage Recommendations | 1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC |
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Storage & Safety
| Storage | Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
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