Chicken IgG (Fab'2) (HRP) (65C-CH0309)
Purified Chicken IgG (Fab'2) HRP conjugate
Overview
Overview
| Synonyms | Chicken Immunoglobulin G (Fab'2) (HRP) |
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| Species | Chicken |
| Applications | User optimized |
Specifications
| Method of Purification | Chicken IgG (Fab'2) (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
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| Tag/Conjugate | HRP |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin supfate. Immunoglobulin and protease free. |
Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope — the antigen-binding site — at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. Treatment with pepsin produces an F(ab)'2 fragment. |
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