cJun antibody (70R-49953)

Purified Polyclonal cJun antibody

Synonyms Polyclonal cJun antibody, Anti-cJun antibody, Transcription factor AP-1 antibody, Activator protein 1 antibody, AP1 antibody, Proto-oncogene c-Jun antibody, V-jun avian sarcoma virus 17 oncogene homolog antibody, p39 antibody
Specificity Recognizes endogenous levels of c-Jun protein
Cross Reactivity Mouse, Rat, Bovine, Chicken, Pig
Applications ChIP, FC, IHC, IP, WB
Immunogen cJun antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-Jun as the immunogen

Images

Western blot analysis using cJun antibody (70R-49953)

Western blot analysis of c-Jun expression in HeLa (A); U2OS (B); HCT116 (C); NIH3T3 (D); PC12 (E); rat kidney (F) whole cell lysates.

Specifications

Host Rabbit
Method of Purification cJun antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IP: 1:10 - 1:100, FC: 1:100 - 1:300

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance cJun activation is associated with proliferation and angiogenesis in invasive breast cancer.

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Images

  • 

Western blot analysis using cJun antibody (70R-49953)



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Western blot analysis of c-Jun expression in HeLa (A); U2OS (B); HCT116 (C); NIH3T3 (D); PC12 (E); rat kidney (F) whole cell lysates.
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Immunohistochemical analysis using cJun antibody (70R-49953)



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Immunohistochemical analysis of c-Jun staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunoprecipitation analysis using cJun antibody (70R-49953)



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Immunoprecipitation of c-Jun from 0.5mg HEK293T whole cell extract lysate using 5ug of Anti-c-Jun Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HEK293T whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-c-Jun Antibody.
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ChIP analysis using cJun antibody ( 70R-49953)



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ChIP analysis of Human endothelial cells (EA.hy926) incubated for 10 hours at 4 C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Position 89340150-89340297 in chromosome 11 (has a validated c-Jun site). Negative Control: Igr5 intron 3 (contains no c-Jun binding site). Detection step: Real-time PCR.

Availability: In stock

Price: €197.96
Size: 100 ul
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