Clusterin antibody (10R-1053)

Mouse monoclonal Clusterin antibody

Synonyms Monoclonal Clusterin antibody, Anti-Clusterin antibody, ApoJ antibody, apolipoprotein J antibody, complement lysis inhibitor antibody, 40 antibody, Clusterin antibody, SGP-2 antibody, ulfated glycoprotein 2 antibody, clusterin (complement lysis inhibitor SP-40 antibody, SP-40 antibody, sulfated glycoprotein 2 testosterone-repressed prostate message 2 apolipoprotein J) antibody, testosterone-repressed prostate message 2 antibody, KUB1 antibody, TRPM-2 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen Clusterin antibody was raised in mouse using recombinant human Clusterin (1-333aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Clusterin antibody (10R-1053)

Cell lysates of U87MG(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 1A11
Isotype IgG1 kappa
Source Anti-human Clusterin antibody, clone 1A11, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Clusterin protein.
Method of Purification Clusterin antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:100-1:200, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Clusterin has been implicated in numerous processes including active cell death, immune regulation, cell adhesion and morphological transformation. Clusterin (previousiy named SP-40) is a glycoprotein, 80kDa comprising two distinct 40kDa polypeptide chains. It shows marked similarity, both physicochemically and in immunohistological distribution, to the single chain, 80kDa, Sprotein, an additional component of the SC5b-9 complex. Clusterin is a secreted glycoprotein of which the exact function remains a matter for speculation.

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Images

  • Western Blot analysis of Clusterin antibody (10R-1053) | Cell lysates of U87MG(20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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