CMV gB protein (30R-AC013)
Purified recombinant CMV gB protein
Overview
Overview
| Synonyms | Cytomegalovirus glycoprotein B protein |
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| Species | Viral |
| Protein Type | Recombinant |
| Applications | ELISA, WB |
Specifications
| Residues | The E.Coli derived recombinant artificial mosaic protein contains the CMV gB immunodominant regions 11-67 amino acids, fused with a 26 kDa GST Tag. |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Method of Purification | CMV gB protein was purified by a proprietary chromatographic technique. |
| Tag/Conjugate | GST tag |
| Form & Buffer | 25mM tris-HCl, pH 8.0, with 1mM EDTA and 50% glycerol. Please Note: Buffer may change with new Lots. |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | CMV belongs to the Beta herpes virinae subfamily of Herpes viridae which includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus. The herpes viruses share a characteristic ability to remain latentover long periods.CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts. |
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