CMV pp150 protein (His tag) (80-1368)

Purified recombinant CMV pp150 protein (His tag)

Synonyms UL32 protein, Cytomegalovirus pp150 protein, Cytomegalovirus UL32 protein, CMV HHV 5 protein, Human herpesvirus 5 protein, Protein UL150, UL150 protein
Species Viral
Protein Type Recombinant
Applications ELISA, WB

Specifications

Residues pp150 expressed as a recombinant antigen fused to a his-tag in its N-terminal. Produced from the ORF of the UL 32 gene which codifies the aa (404-1048)
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 70.825 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea
Concentration 0.5 - 1 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:1000
Bioactivity Immunoreactive with sera of CMV-infected individuals with IgM antibodies

Storage & Safety

Storage Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles

General Information

Biological Significance pp150 (UL32) is a basic phosphoprotein, one of the matrix components. It has been described as highly immunogenic, apparently more so than any other of the HCMV structural proteins.

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