CMV Pp65 protein (30R-AC043)

Purified recombinant CMV Pp65 protein

Synonyms UL83 protein, CMV Pp 65 protein, Cytomegalovirus pp65 Tegument Protein (UL83), CMV pp65 UL83 protein, CMV Pp-65 protein, Cytomegalovirus Pp65 protein, CMV Pp-65, CMV Pp 65, CMV Pp65
Species Viral
Protein Type Recombinant
Applications ELISA, WB

Specifications

Residues The E.Coli derived 52.2 kDa recombinant protein contains the CMV Pp65 (UL83) immunodominant regions, 297-510 amino acids. Recombinant CMV-Pp65 is fused to a 26 kDa GST tag.
Expression System E.coli
Grade & Purity > 95% pure
Method of Purification CMV Pp65 protein was purified by a proprietary chromatographic technique.
Tag/Conjugate GST tag
Form & Buffer 25mM tris-HCl, pH 8.0, with 1mM EDTA and 50% glycerol.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CMV belongs to the Beta herpes virinae subfamily of Herpes viridae which includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus. The herpes viruses share a characteristic ability to remain latentover long periods.CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts.

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